Production of SV-40 Complement-fixing Antigen
Identifieur interne : 000F13 ( Main/Exploration ); précédent : 000F12; suivant : 000F14Production of SV-40 Complement-fixing Antigen
Auteurs : Klaus R. Schell [États-Unis]Source :
- Nature [ 0028-0836 ] ; 1964-07-25.
Abstract
MAYER et al.1 describe the preparation of complement-fixing antigen of vacuolating agent SV-40 in monkey kidney cell cultures. Strain No. 776 of SV -40 used for the work described here was obtained from Dr. H. M. Meyer. In our attempts to prepare a satisfactory complement-fixing antigen, two types of cell cultures were used: primary African green monkey kidney (AGMK) and serially propagated BSCl (ref. 1). These cultures were obtained from Microbiological Associates, Inc., and maintained at 34 C on Eagle's basal medium containing 2 per cent agamma ealf serum, 100 mg of streptomycin per ml., 100 units of penicillin per ml. and 2 mM of glutamine per ml.
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DOI: 10.1038/203417b0
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